五月丁香六月婷婷综合,亚洲欧美日韩台湾色无极,无套中出丰满人妻无码香港,性aa无码专区

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人正常細(xì)胞>CRL-11233™THLE-3 人肝永生化細(xì)胞

THLE-3 人肝永生化細(xì)胞

簡要描述:CRL-11233™ THLE-3 人肝永生化細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件

  • 產(chǎn)品型號:CRL-11233™
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2025-04-27
  • 訪  問  量:1917

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-11233™ THLE-3 人肝永生化細(xì)胞

traces of serum which contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually with 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.  Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4.Add 0.1% Soybean Trypsin inhibitor  and aspirate cells by gently pipetting.

5.To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximay 125 x g for 5 to 10 minutes.

6.Discard supernatant and resuspend cells in fresh growth medium.  Add appropriate aliquots of cell suspension to new coated culture vessels.

7.Place culture vessels in incubators at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:6 is recommended

Medium Renewal: Every 2 to 3 days

CRL-11233™ THLE-3 人肝永生化細(xì)胞

Flask Coating

1.Prepare a mixture of 0.01 mg/mL fibronectin, 0.03 mg/mL bovine collagen type I and 0.01 mg/mL bovine serum albumin (BSA) dissolved in culture medium. Store pre-prepared Coating Solution at 4°C in cold room for up to 3 months.

2.For a growth area of 75 cm2, add 4.5 mL of the fibronectin/collagen/BSA solution and rock gently to coat the entire surface.

3.Incubate the freshly coated vessel(s) in a 37°C incubator overnight (it is preferable to use tissue culture vessels with tightened, plug-seal caps to prevent evaporation during the coating process).

4.Store coated flasks with solution at room temperature, light protected, up to 1 month. Suction off solution before plating cells.


Cryopreservation  Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Culture Conditions  Temperature: 37°C

STR Profile  Amelogenin: X

CSF1PO: 11,12

D13S317: 13

D16S539: 11,12

D5S818: 13

D7S820: 8,10

THO1: 8,9.3

TPOX: 6,9

vWA: 17,18

Name of Depositor  National Cancer Institute

References  Harris CC, et al. Human liver epithelial cells. US Patent 5,759,765 dated Jun 2 1998

Pfeifer AM, et al. Simian virus 40 large tumor antigen-immortalized normal human liver epithelial cells express hepatocyte characteristics and metabolize chemical carcinogens. Proc. Natl. Acad. Sci. USA 90: 5123-5127, 1993. PubMed: 7685115

Pfeifer AM, et al. Simian virus 40 large tumor antigen-immortalized normal human liver epithelial cells express hepatocyte characteristics and metabolize chemical carcinogens. Proc. Natl. Acad. Sci. USA 90: 5123-5127, 1993. PubMed: 7685115

References  Harris CC, et al. Human liver epithelial cells. US Patent 5,759,765 dated Jun 2 1998

Pfeifer AM, et al. Simian virus 40 large tumor antigen-immortalized normal human liver epithelial cells express hepatocyte characteristics and metabolize chemical carcinogens. Proc. Natl. Acad. Sci. USA 90: 5123-5127, 1993. PubMed: 7685115

Pfeifer AM, et al. Simian virus 40 large tumor antigen-immortalized normal human liver epithelial cells express hepatocyte characteristics and metabolize chemical carcinogens. Proc. Natl. Acad. Sci. USA 90: 5123-5127, 1993. PubMed: 7685115

CRL-11233™















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
双腿张开被9个黑人调| 亚洲欧美综合一区二区| 亚洲AV永久无码国产精品久久| 日韩欧美自慰| 亚洲一区91制作| 强奸中年妇女一区二区| 最新国产在线不卡AV| 婷婷基地久久| 国产精品激情无码视频小说| 亚洲、日韩、欧美、国产| 亚洲欧美综合久久| 香蕉尹人在线电影| 国产99久久99热这里只有精品15| 久久99热精品影院| 99视频这里只有精品视频| 制服丝袜在线第一区第二区| 亚洲麻豆h| 深夜免费无码福利视频| 日本无遮挡边做边爱| 草久首页| 99久久国产综合精品1| 国语自产精品视频在线观看 | 午夜国产在线视频| 韩国黄色网站超黄免费| 久久双飞少妇| 中文成人无码精品久久久| 亚洲精品国产制服丝袜美腿| 久久av无码精品人妻系列麻豆| 67194熟妇在线观看线路1| 久久五月丁香网| 日韩一区国产二区不卡| 日本大香伊蕉一区二区久久| 欧美综合干| 免费进入观看色多多| 精品久久亚洲综合| 吞精国产精品久久久久| 亚洲制服丝袜中文无码| 免费无码高潮流白浆视频| 毛都没有就被开了视频苞| 欧美人人揉人人妻人人| 色欲无码人妻AⅤ一区二区三区|