五月丁香六月婷婷综合,亚洲欧美日韩台湾色无极,无套中出丰满人妻无码香港,性aa无码专区

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>CRL-2149SK-N-DZ 人成神經(jīng)瘤細(xì)胞-骨髓

SK-N-DZ 人成神經(jīng)瘤細(xì)胞-骨髓

簡(jiǎn)要描述:CRL-2149 SK-N-DZ 人成神經(jīng)瘤細(xì)胞-骨髓,
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞;細(xì)胞庫(kù)管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-2149
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2025-04-26
  • 訪  問(wèn)  量:2322

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-2149 SK-N-DZ 人成神經(jīng)瘤細(xì)胞-骨髓

ATCC® Number:CRL-2149™  Price:$338.00
Desisgnations:SK-N-DZDepositor:C HelsonBiosafety Level:1Shipped:frozenMedium & Serum:See PropagationGrowth Properties:adherentOrganism:Homo sapiens (human)Morphology:epithelial Source:Organ: brainDisease: neuroblastomaDerived from metastatic site: bone marrowCell Type: neuroblast;Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.CRL-2149 SK-N-DZ 人成神經(jīng)瘤細(xì)胞-骨髓Tumorigenic:YesDNA Profile (STR):Amelogenin: XCSF1PO: 12D13S317: 8,11D16S539: 9,11D5S818: 12D7S820: 12,13THO1: 6,9TPOX: 8vWA: 16,18Cytogenetic Analysis:modal number = 44, XX; most cells were monosomic for chromosomes 10, 11, 13, 14 and 19; all cells were missing both copies of chromosome 2; five marker chromosomes of unknown origin were observed in each cell. One of the marker chromosomes contains a homogeneously staining region (HSR); no double minutes were seenAge:2 yearsGender:femaleEthnicity:CaucasianComments:SK-N-DZ is a neuroblastoma cell line derived in 1978 from a bone marrow metastasis from a child with poorly differentiated embryonal neuroblastoma.Retinoic acid induces differentiation in this line.Expression of the N-myc gene product was reduced in differentiated SK-N-DZ cells as compared with undifferentiated cells.Expression of the c-src gene product, pp60c-src was enhanced in differentiated SK-N-DZ cells as was tyrosine phosphorylation of cellular proteins.The cells exhibit moderate MDR1 expression.Propagation:ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.1 mM nonessential amino acids, 90%; fetal bovine serum, 10%Temperature: 37.0°CSubculturing:Protocol: Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37°C.Subc*tion Ratio: A subc*tion ratio of 1:4 is recommendedMedium Renewal: Every 2 to 3 daysPreservation:Freeze medium: Complete growth medium, 95%; DMSO, 5%Storage temperature: liquid nitrogen vapor phaseRelated Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002recommended serum:ATCC 30-2020References:22290: Sugimoto T, et al. Determination of cell surface membrane antigens common to both human neuroblastoma and leukemia-lymphoma cell lines by a panel of 38 monoclonal antibodies. J. Natl. Cancer Inst. 73: 51-57, 1984. PubMed: 661079222439: Iavarone A, et al. Uptake and storage of m-iodobenzylguanidine are frequent neuronal functions of human neuroblastoma cell lines. Cancer Res. 53: 304-309, 1993. PubMed: 841782423127: Matsumoto M, et al. Expression of proto-oncogene products during drug-induced differentiation of a neuroblastoma cell line SK-N-DZ. Acta Neuropathol. 79: 217-221, 1989. PubMed: 2480694Related Links NCBI Entrez SearchMake a DepositFrequently Asked QuestionsMaterial Transfer AgreementTechnical SupportRelated Cell Culture Products

ATCC® Number: CRL-2149™ Price: $338.00

Designations: SK-N-DZ

Depositors: C Helson

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial

Source: Organ: brain

Disease: neuroblastoma

Derived from metastatic site: bone marrow

Cell Type: neuroblast;

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X

CSF1PO: 12

D13S317: 8,11

D16S539: 9,11

D5S818: 12

D7S820: 12,13

THO1: 6,9

TPOX: 8

vWA: 16,18

Cytogenetic Analysis: modal number = 44, XX; most cells were monosomic for chromosomes 10, 11, 13, 14 and 19; all cells were missing both copies of chromosome 2; five marker chromosomes of unknown origin were observed in each cell. One of the marker chromosomes contains a homogeneously staining region (HSR); no double minutes were seen

Age: 2 years

Gender: female

Ethnicity: Caucasian

Comments: SK-N-DZ is a neuroblastoma cell line derived in 1978 from a bone marrow metastasis from a child with poorly differentiated embryonal neuroblastoma.

Retinoic acid induces differentiation in this line.

Expression of the N-myc gene product was reduced in differentiated SK-N-DZ cells as compared with undifferentiated cells.

Expression of the c-src gene product, pp60c-src was enhanced in differentiated SK-N-DZ cells as was tyrosine phosphorylation of cellular proteins.

The cells exhibit moderate MDR1 expression.

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.1 mM nonessential amino acids, 90%; fetal bovine serum, 10%

Temperature: 37.0°C

Subculturing: Protocol:

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:4 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 22290: Sugimoto T, et al. Determination of cell surface membrane antigens common to both human neuroblastoma and leukemia-lymphoma cell lines by a panel of 38 monoclonal antibodies. J. Natl. Cancer Inst. 73: 51-57, 1984. PubMed: 6610792

22439: Iavarone A, et al. Uptake and storage of m-iodobenzylguanidine are frequent neuronal functions of human neuroblastoma cell lines. Cancer Res. 53: 304-309, 1993. PubMed: 8417824

23127: Matsumoto M, et al. Expression of proto-oncogene products during drug-induced differentiation of a neuroblastoma cell line SK-N-DZ. Acta Neuropathol. 79: 217-221, 1989. PubMed: 2480694

Related Links

NCBI Entrez Search

Make a Deposit

Frequently Asked Questions

Material Transfer Agreement

Technical Support

Related Cell Culture Products






















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
亚洲一本大道av久在线播放| av 这里只有精品| 无码人妻丰满熟妇啪啪站| 欧美三日本三级少妇三99百度| 亚洲欧美大片一区二区三区| 在线 国产 日韩| 亚洲V日韩V精品v无码专区| 调教奶头视频| 出轨人妻日本在线| 国产一区二区三区婬片| av片无码电影在线免费| 久久国内精品自在自线图片| 亚洲激情| 日韩熟妇XXXX| 亚洲精品二区在线www社区| 午夜无码资源| 成人免费看吃奶视频网站| 色欧美成人精品a∨在线观看| 久久www免费人成看片色| 日韩欧洲精品无码AV| 国产最新AV| 色呦呦中文| 亚洲性区青青草| 亚洲精品乱码久久久久久按摩高清| 3d动漫一区二区h| 美韩日综合| 精品蜜臀| 久久久久国产熟女精品| 精品乱子伦一区二区三区电影| 国产精品污污在线观看网站| 国产精品视频导航| 日中国女人屄| 无码理论在线中文字幕 | 色性一区二区三区AV| 色丁香在线观看| 福利在线观看1| 国产在线AⅤ精品| 国产精品区免费在线观看| 最新奇米久久| 亚洲日韩久久AV无码| 91高清国产精品|